Antitubercular activity of Arctium lappa and Tussilago farfara extracts and constituents
Zhao, J. and Evangelopoulos, Dimitrios and Bhakta, Sanjib and Gray, A.I. and Seidel, V. (2014) Antitubercular activity of Arctium lappa and Tussilago farfara extracts and constituents. Journal of Ethnopharmacology 155 (1), pp. 796-800. ISSN 0378-8741.
Abstract
- Ethnopharmacological relevance: Arctium lappa and Tussilago farfara (Asteraceae) are two plant species used traditionally as antitubercular remedies. - Aim: The aim of this study was i) to screen A. lappa and T. farfara extracts for activity against Mycobacterium tuberculosis and ii) to isolate and identify the compound(s) responsible for this reputed anti-TB effect. - Materials and methods: The activity of extracts and isolated compounds was determined against Mycobacterium tuberculosis H37Rv using a high throughput spot culture growth inhibition (HT-SPOTi) assay. - Results: The n-hexane extracts of both plants, the ethyl acetate extract of T. farfara and the dichloromethane phase derived from the methanol extract of A. lappa displayed antitubercular activity (MIC 62.5 μg/mL). Further chemical investigation of A. lappa led to the isolation of n-nonacosane (1), taraxasterol acetate (2), taraxasterol (3), a (1:1) mixture of β sitosterol/stigmasterol (4), isololiolide (5), melitensin (6), trans-caffeic acid (7), kaempferol (8), quercetin (9), kaempferol-3-O-glucoside (10). Compounds isolated from T. farfara were identified as a (1:1) mixture of β sitosterol/stigmasterol (4), trans-caffeic acid (7), kaempferol (8), quercetin (9), kaempferol-3-O-glucoside (10), loliolide (11), a (4:1) mixture of p-coumaric acid/4-hydroxybenzoic acid (12), p-coumaric acid (13). All compounds were identified following analyses of their physicochemical and spectroscopic data (MS, 1H and 13C-NMR) and by comparison with published data. This is the first report of the isolation of n-nonacosane (1), isololiolide (5), melitensin (6) and kaempferol-3-O-glucoside (10) from A. lappa, and of loliolide (11) from T. farfara. Amongst the isolated compounds, the best activity was observed for p-coumaric acid (13) (MIC 31.3 μg/mL or 190.9 μM) alone and in mixture with 4-hydroxybenzoic acid (12) (MIC 62.5 μg/mL). - Conclusions: The above results provide for the first time some scientific evidence to support, to some extent, the ethno-medicinal use of A. lappa and T. farfara as traditional antitubercular remedies.
Metadata
| Item Type: | Article |
|---|---|
| Keyword(s) / Subject(s): | Antitubercular activity, Arctium lappa, Tussilago farfara, Mycobacterium tuberculosis |
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Research Centres and Institutes: | Structural Molecular Biology, Institute of (ISMB) |
| Depositing User: | Administrator |
| Date Deposited: | 23 Jun 2014 09:25 |
| Last Modified: | 02 Aug 2023 17:11 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/10008 |
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