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    Compartmentalized self-tagging for in vitro-directed evolution of XNA polymerases

    Pinheiro, Vitor B. and Arangundy-Franklin, S. and Holliger, P. (2014) Compartmentalized self-tagging for in vitro-directed evolution of XNA polymerases. Current Protocols In Nucleic Acid Chemistry 57 , 9.9.1-9.9.18.

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    Abstract

    Template-dependent synthesis of xenobiotic nucleic acids (XNAs) is an essential step for the development of functional XNA molecules, as it enables Darwinian evolution to be carried out with novel genetic polymers. The extraordinary substrate specificity of natural DNA polymerases greatly restricts the spectrum of XNAs available, thus making it necessary to identify DNA polymerase variants capable of incorporating a wider range of substrates. This unit summarizes compartmentalized self-tagging (CST), a directed evolution strategy developed for the selection of DNA polymerase variants capable of XNA synthesis.

    Metadata

    Item Type: Article
    Additional Information: ISBN: 9780471142706
    Keyword(s) / Subject(s): DNA polymerases, in vitro-directed evolution, synthetic nucleic acids, XNA, compartmentalized self-tagging, CST
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 01 Jul 2014 09:11
    Last Modified: 06 Dec 2016 11:17
    URI: http://eprints.bbk.ac.uk/id/eprint/10040

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