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    Structure of a C. perfringens Enterotoxin mutant in complex with a Modified Claudin-2 Extracellular Loop 2

    Yelland, T.S. and Naylor, Claire E. and Bagoban, T. and Savva, Christos G. and Moss, David S. and McClane, B.A. and Blasig, I.E. and Popoff, M. and Basak, Ajit K. (2014) Structure of a C. perfringens Enterotoxin mutant in complex with a Modified Claudin-2 Extracellular Loop 2. Journal of Molecular Biology 426 (18), pp. 3134-3147. ISSN 0022-2836.

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    Abstract

    CPE (Clostridium perfringens enterotoxin) is the major virulence determinant for C. perfringens type A food poisoning, the second most common bacterial food-borne illness in the UK and USA. After binding to its receptors, which include particular human claudins, the toxin forms pores in the cell membrane. The mature pore apparently contains a hexamer of CPE, claudin and, possibly, occludin. The combination of high binding specificity with cytotoxicity has resulted in CPE being investigated, with some success, as a targeted cytotoxic agent for oncotherapy. In this paper, we present the X-ray crystallographic structure of CPE in complex with a peptide derived from extracellular loop 2 of a modified, CPE binding Claudin-2, together with high-resolution native and pore-formation mutant structures. Our structure provides the first atomic resolution data on any part of a claudin molecule and reveals that claudin’s CPE binding fingerprint (NPLVP), is in a tight turn conformation and binds, as expected, in CPE’s C-terminal claudin binding groove. The leucine and valine residues insert into the binding groove while the first residue, asparagine, tethers the peptide via an interaction with CPE’s aspartate 225 and the two prolines are required to maintain the tight turn conformation. Understanding the structural basis of the contribution these residues make to binding will aid in engineering CPE to target tumour cells.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): enterotoxin, claudin, receptor, complex, structure
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 21 Jul 2014 10:28
    Last Modified: 06 Dec 2016 10:43
    URI: http://eprints.bbk.ac.uk/id/eprint/10197

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