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    A conserved domain targets exported PHISTb family proteins to the periphery of Plasmodium infected erythrocytes

    Tarr, Sarah J. and Moon, R.W. and Hardege, Iris and Osborne, Andrew R. (2014) A conserved domain targets exported PHISTb family proteins to the periphery of Plasmodium infected erythrocytes. Molecular and Biochemical Parasitology 196 (1), pp. 29-40. ISSN 0166-6851.

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    Abstract

    During blood-stage infection, malaria parasites export numerous proteins to the host erythrocyte. The Poly-Helical Interspersed Sub-Telomeric (PHIST) proteins are an exported family that share a common ‘PRESAN’ domain, and include numerous members in Plasmodium falciparum, Plasmodium vivax and Plasmodium knowlesi. In P. falciparum, PHIST proteins have been implicated in protein trafficking and intercellular communication. A number of PHIST proteins are essential for parasite survival. Here, we identify nine members of the PHISTb sub-class of PHIST proteins, including one protein known to be essential for parasite survival, that localise to the erythrocyte periphery. These proteins have solubility characteristics consistent with their association with the erythrocyte cytoskeleton. Together, an extended PRESAN domain, comprising the PRESAN domain and preceding sequence, form a novel targeting-domain that is sufficient to localise a protein to the erythrocyte periphery. We validate the role of this domain in RESA, thus identifying a cytoskeleton-binding domain in RESA that functions independently of its known spectrin-binding domain. Our data suggest that some PHISTb proteins may act as cross-linkers of the erythrocyte cytoskeleton. We also show for the first time that peripherally-localised PHISTb proteins are encoded in genomes of P. knowlesi and vivax indicating a conserved role for the extended PRESAN domain of these proteins in targeting to the erythrocyte periphery

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): PHIST, PRESAN, Plasmodium, Cytoskeleton, Protein export, Malaria
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 12 Aug 2014 08:19
    Last Modified: 06 Dec 2016 11:16
    URI: http://eprints.bbk.ac.uk/id/eprint/10391

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