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Identification of the HLA-DM/HLA-DR interface

Davies, M.N. and Lamikanra, A. and Sansom, Clare E. and Flower, D.R. and Moss, David S. and Travers, P.J. (2008) Identification of the HLA-DM/HLA-DR interface. Molecular Immunology 45 (4), pp. 1063-1070. ISSN 0161-5890.

Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.molimm.2007.07.033

Abstract

Human leukocyte antigen (HLA)-DM is a critical participant in antigen presentation that catalyzes the dissociation of the Class II-associated Invariant chain-derived Peptide (CLIP) from the major histocompatibility complex (MHC) Class II molecules. There is competition amongst peptides for access to an MHC Class II groove and it has been hypothesised that DM functions as a ‘peptide editor’ that catalyzes the replacement of one peptide for another within the groove. It is established that the DM catalyst interacts directly with the MHC Class II but the precise location of the interface is unknown. Here, we combine previously described mutational data with molecular docking and energy minimisation simulations to identify a putative interaction site of >4000 Å2 which agrees with known point mutational data for both the DR and DM molecule. The docked structure is validated by comparison with experimental data and previously determined properties of protein–protein interfaces. A possible dissociation mechanism is suggested by the presence of an acidic cluster near the N terminus of the bound peptide.

Item Type: Article
School or Research Centre: Birkbeck Schools and Research Centres > School of Science > Biological Sciences
Depositing User: Administrator
Date Deposited: 04 Aug 2010 14:09
Last Modified: 17 Apr 2013 12:17
URI: http://eprints.bbk.ac.uk/id/eprint/1048

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