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    Basal foot MTOC organizes pillar MTs required for coordination of beating cilia

    Clare, Daniel K. and Magescas, J. and Piolot, T. and Dumoux, Maud and Vesque, C. and Pichard, E. and Dang, T. and Duvauchelle, B. and Poirier, F. and Delacour, D. (2014) Basal foot MTOC organizes pillar MTs required for coordination of beating cilia. Nature Communications 5 , p. 4888. ISSN 2041-1723.

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    Abstract

    Coordination of ciliary beating is essential to ensure mucus clearance in the airway tract. The orientation and synchronization of ciliary motion responds in part to the organization of the underlying cytoskeletal networks. Using electron tomography on mouse trachea, we show that basal bodies are collectively hooked at the cortex by a regular microtubule array composed of 4–5 microtubules. Removal of ​galectin-3, one of basal-body components, provokes misrecruitment of γ-tubulin, disorganization of this microtubule framework emanating from the basal-foot cap, together with loss of basal-body alignment and cilium orientation, defects in cilium organization and reduced fluid flow in the tracheal lumen. We conclude that ​galectin-3 plays a crucial role in the maintenance of the microtubule-organizing centre of the cilium and the ‘pillar’ microtubules, and that this network is instrumental for the coordinated orientation and stabilization of motile cilia.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): Biological sciences, Cell biology
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Depositing User: Administrator
    Date Deposited: 15 Sep 2014 09:19
    Last Modified: 15 Sep 2014 09:19
    URI: http://eprints.bbk.ac.uk/id/eprint/10517

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