Structure of the mammalian 80S ribosome at 87 angstrom resolution
Chandramouli, P. and Topf, Maya and Menetret, J.F. and Eswar, N. and Cannone, J.J. and Gutell, R.R. and Sali, A. and Akey, C.W. (2008) Structure of the mammalian 80S ribosome at 87 angstrom resolution. Structure 16 (4), pp. 535-548. ISSN 0969-2126.
In this paper, we present a structure of the mammalian ribosome determined at not, vert, similar8.7 Å resolution by electron cryomicroscopy and single-particle methods. A model of the ribosome was created by docking homology models of subunit rRNAs and conserved proteins into the density map. We then modeled expansion segments in the subunit rRNAs and found unclaimed density for not, vert, similar20 proteins. In general, many conserved proteins and novel proteins interact with expansion segments to form an integrated framework that may stabilize the mature ribosome. Our structure provides a snapshot of the mammalian ribosome at the beginning of translation and lends support to current models in which large movements of the small subunit and L1 stalk occur during tRNA translocation. Finally, details are presented for intersubunit bridges that are specific to the eukaryotic ribosome. We suggest that these bridges may help reset the conformation of the ribosome to prepare for the next cycle of chain elongation.
|Keyword(s) / Subject(s):||RNA|
|School:||Birkbeck Schools and Departments > School of Science > Biological Sciences|
|Research Centre:||Bioinformatics, Bloomsbury Centre for, Structural Molecular Biology, Institute of (ISMB)|
|Date Deposited:||04 Aug 2010 14:09|
|Last Modified:||07 Dec 2016 15:02|
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