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Comprehensive molecular structure of the Eukaryotic Ribosome

Taylor, D.J. and Devkota, B. and Huang, A.D. and Topf, Maya and Narayanan, E. and Sali, A. and Harvey, S.C. and Frank, J. (2009) Comprehensive molecular structure of the Eukaryotic Ribosome. Structure 17 (12), pp. 1591-1604. ISSN 0969-2126.

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Official URL: http://dx.doi.org/10.1016/j.str.2009.09.015

Abstract

Despite the emergence of a large number of X-ray crystallographic models of the bacterial 70S ribosome over the past decade, an accurate atomic model of the eukaryotic 80S ribosome is still not available. Eukaryotic ribosomes possess more ribosomal proteins and ribosomal RNA than do bacterial ribosomes, which are implicated in extraribosomal functions in the eukaryotic cells. By combining cryo-EM with RNA and protein homology modeling, we obtained an atomic model of the yeast 80S ribosome complete with all ribosomal RNA expansion segments and all ribosomal proteins for which a structural homolog can be identified. Mutation or deletion of 80S ribosomal proteins can abrogate maturation of the ribosome, leading to several human diseases. We have localized one such protein unique to eukaryotes, rpS19e, whose mutations are associated with Diamond-Blackfan anemia in humans. Additionally, we characterize crucial interactions between the dynamic stalk base of the ribosome with eukaryotic elongation factor 2.

Item Type: Article
Keyword(s) / Subject(s): Proteins, RNA, DNA
School or Research Centre: Birkbeck Schools and Research Centres > School of Science > Biological Sciences
Depositing User: Administrator
Date Deposited: 04 Aug 2010 14:09
Last Modified: 17 Apr 2013 12:17
URI: http://eprints.bbk.ac.uk/id/eprint/1149

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