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Fiber formation across the bacterial outer membrane by the chaperone/usher pathway

Remaut, Han and Tang, C. and Henderson, N.S. and Pinkner, J.S. and Wang, T. and Hultgren, S.J. and Thanassi, D.G. and Waksman, Gabriel and Li, H.L. (2008) Fiber formation across the bacterial outer membrane by the chaperone/usher pathway. Cell 133 (4), pp. 640-652. ISSN 0092-8674.

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Official URL: http://dx.doi.org/10.1016/j.cell.2008.03.033

Abstract

Gram-negative pathogens commonly exhibit adhesive pili on their surfaces that mediate specific attachment to the host. A major class of pili is assembled via the chaperone/usher pathway. Here, the structural basis for pilus fiber assembly and secretion performed by the outer membrane assembly platform—the usher—is revealed by the crystal structure of the translocation domain of the P pilus usher PapC and single particle cryo-electron microscopy imaging of the FimD usher bound to a translocating type 1 pilus assembly intermediate. These structures provide molecular snapshots of a twinned-pore translocation machinery in action. Unexpectedly, only one pore is used for secretion, while both usher protomers are used for chaperone-subunit complex recruitment. The translocating pore itself comprises 24 β strands and is occluded by a folded plug domain, likely gated by a conformationally constrained β-hairpin. These structures capture the secretion of a virulence factor across the outer membrane of Gram-negative bacteria.

Item Type: Article
Keyword(s) / Subject(s): microbio, cellbio
School or Research Centre: Birkbeck Schools and Research Centres > School of Science > Biological Sciences
Depositing User: Administrator
Date Deposited: 04 Aug 2010 14:09
Last Modified: 17 Apr 2013 12:17
URI: http://eprints.bbk.ac.uk/id/eprint/1160

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