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    Importin-β modulates the permeability of the nuclear pore complex in a Ran-dependent manner

    Lowe, Alan R. and Tang, J.H and Yassif, J. and Graf, M. and Huang, W.Y.C. and Groves, J.T. and Weis, K. and Liphardt, J.T. (2015) Importin-β modulates the permeability of the nuclear pore complex in a Ran-dependent manner. eLife 4 , ISSN 2050-084X.

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    Abstract

    Soluble karyopherins of the importin-β (impβ) family use RanGTP to transport cargos directionally through the nuclear pore complex (NPC). Whether impβ or RanGTP regulate the permeability of the NPC itself has been unknown. Here, we identify a stable pool of impβ at the NPC. A subpopulation of this pool is rapidly turned-over by RanGTP, likely at Nup153. Impβ, but not transportin-1 (TRN1), alters the pore's permeability in a Ran-dependent manner, suggesting that impβ is a functional component of the NPC. Upon reduction of Nup153 levels, inert cargos more readily equilibrate across the NPC yet active transport is impaired. When purified impβ or TRN1 are mixed with Nup153 in vitro, higher-order, multivalent complexes form. RanGTP dissolves the impβ•Nup153 complexes but not those of TRN1•Nup153. We propose that impβ and Nup153 interact at the NPC's nuclear face to form a Ran-regulated mesh that modulates NPC permeability.

    Metadata

    Item Type: Article
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 11 Mar 2015 09:02
    Last Modified: 06 Dec 2016 10:39
    URI: http://eprints.bbk.ac.uk/id/eprint/11820

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