Bagneris, Claire and Ageichik, A.V. and Cronin, Nora and Wallace, Bonnie A. and Collins, M. and Boshoff, C. and Waksman, Gabriel and Barrett, Tracey E. (2008) Crystal structure of a vFlip-IKK gamma complex: Insights into viral activation of the IKK signalosome. Molecular Cell 30 (5), pp. 620-631. ISSN 1097-2765.Full text not available from this repository.
Key to the pathogenicity of several viruses is activation of the canonical nuclear factor-κB (NF-κB) transcriptional pathway. Subversion of this tightly regulated mechanism is achieved through the production of host mimetic viral proteins that deregulate the transcription process. One such protein is ks-vFLIP (produced by the Kaposi's sarcoma herpes virus [KSHV]), which associates with IKKγ, an essential component of the IKK complex or signalosome. This interaction renders the canonical NF-κB pathway constitutively active and has been linked to Kaposi's sarcoma and other malignancies. In order to elucidate the molecular basis underpinning ks-vFLIP-induced activation of the IKK signalosome, we have determined the crystal structure of a complex involving a fragment of IKKγ bound to ks-vFLIP at 3.2 Å. In addition to identifying and subsequently probing the ks-vFLIP-IKKγ interface, we have also investigated the effects of a mutation implicated in the genetic disorder anhydrotic ectodermal dysplasia with immunodeficiency (EDA-ID).
|School or Research Centre:||Birkbeck Schools and Research Centres > School of Science > Biological Sciences|
|Date Deposited:||04 Aug 2010 14:09|
|Last Modified:||17 Apr 2013 12:17|
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