Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines
Miles, Andrew J. and Janes, R.W. and Brown, A. and Clarke, D.T. and Sutherland, J.C. and Tao, Y. and Wallace, Bonnie A. and Hoffmann, S.V. (2008) Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines. Journal of Synchrotron Radiation 15 , pp. 420-422. ISSN 0909-0495.
New high-flux synchrotron radiation circular dichroism (SRCD) beamlines are providing important information for structural biology, but can potentially cause denaturation of the protein samples under investigation. This effect has been studied at the new CD1 dedicated SRCD beamline at ISA in Denmark, where radiation-induced thermal damage effects were observed, depending not only on the radiation flux but also on the focal spot size of the light. Comparisons with similar studies at other SRCD facilities worldwide has lead to the estimation of a flux density threshold under which SRCD beamlines should be operated when samples are to be exposed to low-wavelength vacuum ultraviolet radiation for extended periods of time.
|Keyword(s) / Subject(s):||protein denaturation, radiation damage, synchrotron radiation circular dichroism spectroscopy, photon flux, vacuum ultraviolet wavelengths|
|School:||Birkbeck Schools and Departments > School of Science > Biological Sciences|
|Research Centre:||Bioinformatics, Bloomsbury Centre for, Structural Molecular Biology, Institute of (ISMB)|
|Date Deposited:||04 Aug 2010 14:09|
|Last Modified:||07 Dec 2016 15:05|
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