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    A novel and rapid method for obtaining high titre intact prion strains from mammalian brain

    Wenborn, A. and Terry, C. and Gros, N. and Joiner, S. and D’Castro, L. and Panico, Silvia and Sells, J. and Cronier, S. and Linehan, J.M. and Brandner, S. and Saibil, Helen R. and Collinge, J. and Wadsworth, J.D.F. (2015) A novel and rapid method for obtaining high titre intact prion strains from mammalian brain. Scientific Reports 5 , p. 10062. ISSN 2045-2322.

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    Abstract

    Mammalian prions exist as multiple strains which produce characteristic and highly reproducible phenotypes in defined hosts. How this strain diversity is encoded by a protein-only agent remains one of the most interesting and challenging questions in biology with wide relevance to understanding other diseases involving the aggregation or polymerisation of misfolded host proteins. Progress in understanding mammalian prion strains has however been severely limited by the complexity and variability of the methods used for their isolation from infected tissue and no high resolution structures have yet been reported. Using high-throughput cell-based prion bioassay to re-examine prion purification from first principles we now report the isolation of prion strains to exceptional levels of purity from small quantities of infected brain and demonstrate faithful retention of biological and biochemical strain properties. The method’s effectiveness and simplicity should facilitate its wide application and expedite structural studies of prions.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): Encephalopathy, Neurodegeneration
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 11 May 2015 08:06
    Last Modified: 06 Dec 2016 11:14
    URI: http://eprints.bbk.ac.uk/id/eprint/12089

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