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    Development of a rapid, reliable and quantitative method – “SPOTi” for testing antifungal efficacy

    Rizi, K. and Murdan, S. and Danquah, Cynthia A. and Nicklin, Jane and Bhakta, Sanjib (2015) Development of a rapid, reliable and quantitative method – “SPOTi” for testing antifungal efficacy. Journal of Microbiological Methods 117 , pp. 36-40. ISSN 0167-7012.

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    Abstract

    A reference method for the antimicrobial susceptibility testing of common fungal pathogens such as dermatophytes, is currently lacking. In this study, we report the successful adaptation of solid agar-based spot culture growth inhibition assay (SPOTi) for dermatophytes, currently being used as a gold-standard in the anti-tubercular drug discovery field. The fungal-SPOTi assay correlated with the disc-diffusion method, and is validated using mycelial plugs. We propose the fungal-SPOTi as a high-throughput alternative to the disc-diffusion and broth micro-dilution anti-fungal assays to screen novel anti-fungals.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): SPOTi, antifungals, dermatophytes, screening, drug discovery
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 22 Jul 2015 12:38
    Last Modified: 06 Dec 2016 11:23
    URI: http://eprints.bbk.ac.uk/id/eprint/12552

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