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    Novel inter-subunit contacts in Barley Stripe Mosaic Virus revealed by Cryo-Electron Microscopy

    Clare, Daniel Kofi and Pechnikova, E.V. and Skurat, E.V. and Makarov, V.V. and Sokolova, O.S. and Solovyev, A.G. and Orlova, Elena (2015) Novel inter-subunit contacts in Barley Stripe Mosaic Virus revealed by Cryo-Electron Microscopy. Structure 23 (10), pp. 1815-1826. ISSN 0969-2126.

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    Abstract

    Barley stripe mosaic virus (BSMV, genus Hordeivirus) is a rod-shaped single-stranded RNA virus similar to viruses of the structurally characterized and well-studied genus Tobamovirus. Here we report the first high-resolution structure of BSMV at 4.1 Å obtained by cryo-electron microscopy. We discovered that BSMV forms two types of virion that differ in the number of coat protein (CP) subunits per turn and interactions between the CP subunits. While BSMV and tobacco mosaic virus CP subunits have a similar fold and interact with RNA using conserved residues, the axial contacts between the CP of these two viral groups are considerably different. BSMV CP subunits lack substantial axial contacts and are held together by a previously unobserved lateral contact formed at the virion surface via an interacting loop, which protrudes from the CP hydrophobic core to the adjacent CP subunit. These data provide an insight into diversity in structural organization of helical viruses.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): BSMV, helical virus, Cryo-EM, CCD camera, single particle processing
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 26 Aug 2015 15:21
    Last Modified: 26 Jul 2019 18:02
    URI: http://eprints.bbk.ac.uk/id/eprint/12888

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