BIROn - Birkbeck Institutional Research Online

    Quantification of global transcription patterns in prokaryotes using spotted microarrays

    Sidders, B. and Withers, M. and Kendall, S.L. and Bacon, J. and Waddell, S.J. and Hinds, J. and Golby, P. and Movahedzadeh, F. and Cox, R.A. and Frita, R. and ten Bokum, A.M.C. and Wernisch, Lorenz and Stoker, N.G. (2007) Quantification of global transcription patterns in prokaryotes using spotted microarrays. Genome Biology 8 (12), R265. ISSN 1465-6906.

    [img]
    Preview
    Text
    12941.pdf - Published Version of Record
    Available under License Creative Commons Attribution.

    Download (1MB) | Preview

    Abstract

    We describe an analysis, applicable to any spotted microarray dataset produced using genomic DNA as a reference, that quantifies prokaryotic levels of mRNA on a genome-wide scale. Applying this to Mycobacterium tuberculosis, we validate the technique, show a correlation between level of expression and biological importance, define the complement of invariant genes and analyze absolute levels of expression by functional class to develop ways of understanding an organism's biology without comparison to another growth condition.

    Metadata

    Item Type: Article
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Depositing User: Administrator
    Date Deposited: 10 Sep 2015 11:29
    Last Modified: 02 Aug 2023 17:18
    URI: https://eprints.bbk.ac.uk/id/eprint/12941

    Statistics

    Activity Overview
    6 month trend
    314Downloads
    6 month trend
    205Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item