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    Quantification of global transcription patterns in prokaryotes using spotted microarrays

    Sidders, B. and Withers, M. and Kendall, S.L. and Bacon, J. and Waddell, S.J. and Hinds, J. and Golby, P. and Movahedzadeh, F. and Cox, R.A. and Frita, R. and ten Bokum, A.M.C. and Wernisch, Lorenz and Stoker, N.G. (2007) Quantification of global transcription patterns in prokaryotes using spotted microarrays. Genome Biology 8 (12), R265. ISSN 1465-6906.

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    Abstract

    We describe an analysis, applicable to any spotted microarray dataset produced using genomic DNA as a reference, that quantifies prokaryotic levels of mRNA on a genome-wide scale. Applying this to Mycobacterium tuberculosis, we validate the technique, show a correlation between level of expression and biological importance, define the complement of invariant genes and analyze absolute levels of expression by functional class to develop ways of understanding an organism's biology without comparison to another growth condition.

    Metadata

    Item Type: Article
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Depositing User: Administrator
    Date Deposited: 10 Sep 2015 11:29
    Last Modified: 28 Jul 2019 09:32
    URI: http://eprints.bbk.ac.uk/id/eprint/12941

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