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    Structure of a bacterial type III secretion system in contact with a host membrane in situ

    Nans, Andrea and Kudryashev, M. and Saibil, Helen R. and Hayward, Richard D. (2015) Structure of a bacterial type III secretion system in contact with a host membrane in situ. Nature Communications 6 , p. 1104. ISSN 2041-1723.

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    Abstract

    Many bacterial pathogens of animals and plants use a conserved type III secretion system (T3SS) to inject virulence effector proteins directly into eukaryotic cells to subvert host functions. Contact with host membranes is critical for T3SS activation, yet little is known about T3SS architecture in this state or the conformational changes that drive effector translocation. Here we use cryo-electron tomography and sub-tomogram averaging to derive the intact structure of the primordial Chlamydia trachomatis T3SS in the presence and absence of host membrane contact. Comparison of the averaged structures demonstrates a marked compaction of the basal body (4 nm) occurs when the needle tip contacts the host cell membrane. This compaction is coupled to a stabilization of the cytosolic sorting platform– ATPase. Our findings reveal the first structure of a bacterial T3SS from a major human pathogen engaged with a eukaryotic host, and reveal striking ‘pump-action’ conformational changes that underpin effector injection.

    Metadata

    Item Type: Article
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 06 Jan 2016 14:31
    Last Modified: 09 May 2018 18:13
    URI: http://eprints.bbk.ac.uk/id/eprint/13594

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