Cryo electron microscopy to determine the structure of macromolecular complexes
Carroni, Marta and Saibil, Helen R. (2016) Cryo electron microscopy to determine the structure of macromolecular complexes. Methods 95 , pp. 78-85. ISSN 1046-2023.
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Abstract
Cryo-electron microscopy (cryo-EM) is a structural molecular and cellular biology technique that has experienced major advances in recent years. Technological developments in image recording as well as in processing software make it possible to obtain three-dimensional reconstructions of macromolecular assemblies at near-atomic resolution that were formerly obtained only by X-ray crystallography or NMR spectroscopy. In parallel, cryo-electron tomography has also benefitted from these technological advances, so that visualization of irregular complexes, organelles or whole cells with their molecular machines in situ has reached subnanometre resolution. Cryo-EM can therefore address a broad range of biological questions. The aim of this review is to provide a brief overview of the principles and current state of the cryo-EM field.
Metadata
| Item Type: | Article |
|---|---|
| School: | Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences |
| Research Centres and Institutes: | Structural Molecular Biology, Institute of (ISMB) |
| Depositing User: | Administrator |
| Date Deposited: | 30 Nov 2015 13:31 |
| Last Modified: | 02 Aug 2023 17:20 |
| URI: | https://eprints.bbk.ac.uk/id/eprint/13646 |
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