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    Single-molecule imaging to characterise the transport mechanism of the Nuclear Pore Complex

    Jeremy, G. and Stevens, J. and Lowe, Alan R. (2016) Single-molecule imaging to characterise the transport mechanism of the Nuclear Pore Complex. In: Leake, M.C. (ed.) Chromosome Architecture: Methods and Protocols. Methods in Molecular Biology 1431. New York, U.S.: Springer New York, pp. 17-35. ISBN 9781493936298.

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    Abstract

    In the eukaryotic cell, a large macromolecular channel, known as the Nuclear Pore Complex (NPC), mediates all molecular transport between the nucleus and cytoplasm. In recent years, single-molecule fluorescence (SMF) imaging has emerged as a powerful tool to study the molecular mechanism of transport through the NPC. More recently, techniques such as Single-Molecule Localisation Microscopy (SMLM) have enabled the spatial and temporal distribution of cargos, transport receptors and even structural components of the NPC to be determined with nanometre accuracy. In this protocol, we describe a method to study the position and/or motion of individual molecules transiting through the NPC with high spatial and temporal precision.

    Metadata

    Item Type: Book Section
    Additional Information: Series ISSN: 1064-3745: The original publication is available at www.springerlink.com
    Keyword(s) / Subject(s): Nucleus, Nuclear Pore Complex, Single-Molecule Tracking, Super-resolution Microscopy
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 07 Jul 2016 14:00
    Last Modified: 27 Apr 2018 08:30
    URI: http://eprints.bbk.ac.uk/id/eprint/14935

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