BIROn - Birkbeck Institutional Research Online

    Circular dichroism spectroscopy of membrane proteins

    Miles, Andrew J. and Wallace, Bonnie A. (2016) Circular dichroism spectroscopy of membrane proteins. Chemical Society Reviews 18 , pp. 4859-4872. ISSN 0306-0012.

    [img]
    Preview
    Text
    15667.pdf - Published Version of Record
    Available under License Creative Commons Attribution.

    Download (2MB) | Preview

    Abstract

    Circular dichroism (CD) spectroscopy is a well-established technique for studying the secondary structures, dynamics, folding pathways, and interactions of soluble proteins, and is complementary to the high resolution but generally static structures produced by X-ray crystallography, NMR spectroscopy, and cryo electron microscopy. CD spectroscopy has special relevance for the study of membrane proteins, which are difficult to crystallise and largely ignored in structural genomics projects. However, the requirement for membrane proteins to be embedded in amphipathic environments such as membranes, lipid vesicles, detergent micelles, bicelles, oriented bilayers, or nanodiscs, in order for them to be soluble or dispersed in solution whilst maintaining their structure and function, necessitates the use of different experimental and analytical approaches than those employed for soluble proteins. This review discusses specialised methods for collecting and analysing membrane protein CD data, highlighting where protocols for soluble and membrane proteins diverge.

    Metadata

    Item Type: Article
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Bioinformatics, Bloomsbury Centre for, Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 29 Jun 2016 16:03
    Last Modified: 04 Sep 2017 14:24
    URI: http://eprints.bbk.ac.uk/id/eprint/15667

    Statistics

    Downloads
    Activity Overview
    38Downloads
    77Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item