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    Reaction intermediates and molecular mechanism of Peroxynitrite activation by NO synthases

    Lang, J. and Marechal, Amandine and Couture, M. and Santolini, J. (2016) Reaction intermediates and molecular mechanism of Peroxynitrite activation by NO synthases. Biophysical Journal 111 (10), pp. 2099-2109. ISSN 0006-3495.

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    Abstract

    The activation of peroxynitrite (PN) by hemoproteins, which leads to its detoxification or on the contrary to the enhancement of its cytotoxic activity, is a reaction of physiological importance that is still poorly understood. It has been known for some years that the reaction of hemoproteins, notably cytochrome P450, with PN leads to the build-up of an intermediate species with a Soret band at ~435 nm (I435). The nature of this intermediate is however debated. On the one hand, I435 has been presented as a Compound-II species that can be photo-activated to Compound I. A competing alternative involves the assignment of I435 to a ferric-nitrosyl species. Alike the cytochromes P450, the build-up of I435 occurs in NO-synthases (NOSs) upon their reaction with excess PN. Interestingly, the NOS isoforms vary in their capacity to detoxify/activate PN although they all show the build-up of I435. To better understand PN activation/detoxification by heme proteins, a definitive assignment of I435 is needed. Here we used a combination of fine kinetic analysis under specific conditions (pH, PN concentrations and PN/NOSs ratios) to probe the formation of I435. These studies revealed that I435 is not formed upon homolytic cleavage of the O-O bond of PN but that it arises from side-reactions associated with excess PN. Characterization of I435 by resonance Raman spectroscopy allowed its identification as a ferric iron-nitrosyl complex. Together, our study indicates that the model used so far to depict PN interactions with hemo-thiolate proteins, i.e. leading to the formation and accumulation of Compound II, needs to be reconsidered.

    Metadata

    Item Type: Article
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Depositing User: Dr Amandine Marechal
    Date Deposited: 25 Jan 2017 11:20
    Last Modified: 16 Nov 2017 01:10
    URI: http://eprints.bbk.ac.uk/id/eprint/16759

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