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    Structures of biomolecular complexes by combination of NMR and cryoEM methods

    Cuniasse, P. and Tavares, P. and Orlova, Elena and Zinn-Justin, S. (2017) Structures of biomolecular complexes by combination of NMR and cryoEM methods. Current Opinion in Structural Biology 43 , pp. 104-113. ISSN 0959-440X.

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    Abstract

    CryoEM is presently providing structures of biocomplexes considered intractable to analysis by other structural techniques. NMR is playing an important role in delivering structural information on dynamics events and conformational heterogeneity. Impressive results were obtained by combining cryoEM and either liquid- or solid-state NMR, revealing the structures of cellular machines, filaments and amyloid fibrils. NMR solution structures of proteins and nucleic acids were fitted, together with crystallographic structures, into cryoEM maps of large complexes, to decipher their assembly mechanisms and describe their functional dynamics. Modeling based on solid-state NMR and cryoEM data provided 3D structure of filaments and fibrils. These NMR approaches validated, but also corrected, atomic models built de novo in cryoEM maps, and provided new structural data on flexible or structurally heterogeneous systems. Combination of cryoEM and NMR became an established hybrid approach in structural biology that significantly contributes to our understanding of functional mechanisms in supramolecular assemblies.

    Metadata

    Item Type: Article
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Depositing User: Administrator
    Date Deposited: 23 Jan 2017 11:12
    Last Modified: 03 Jul 2018 00:10
    URI: http://eprints.bbk.ac.uk/id/eprint/17984

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