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    Attenuation of Phosphorylation-dependent activation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by disease-causing mutations at the transmission interface

    Chin, S. and Yang, D. and Miles, Andrew J. and Eckford, P.D.W. and Molinski, S. and Wallace, Bonnie A. and Bear, C.E. (2016) Attenuation of Phosphorylation-dependent activation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by disease-causing mutations at the transmission interface. Journal of Biological Chemistry 292 (5), pp. 1988-1999. ISSN 0021-9258.

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    Abstract

    Cystic fibrosis transmembrane conductance regulator (CFTR) is a multidomain membrane protein that functions as a phosphorylation-regulated anion channel. The interface between its two cytosolic nucleotide binding domains and coupling helices conferred by intracellular loops extending from the channel pore domains has been referred to as a transmission interface and is thought to be critical for the regulated channel activity of CFTR. Phosphorylation of the regulatory domain of CFTR by protein kinase A (PKA) is required for its channel activity. However, it was unclear if phosphorylation modifies the transmission interface. Here, we studied purified full-length CFTR protein using spectroscopic techniques to determine the consequences of PKA-mediated phosphorylation. Synchrotron radiation circular dichroism spectroscopy confirmed that purified full-length wild-type CFTR is folded and structurally responsive to phosphorylation. Intrinsic tryptophan fluorescence studies of CFTR showed that phosphorylation reduced iodide-mediated quenching, consistent with an effect of phosphorylation in burying tryptophans at the transmission interface. Importantly, the rate of phosphorylation-dependent channel activation was compromised by the introduction of disease-causing mutations in either of the two coupling helices predicted to interact with nucleotide binding domain 1 at the interface. Together, these results suggest that phosphorylation modifies the interface between the catalytic and pore domains of CFTR and that this modification facilitates CFTR channel activation.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): cysteine-mediated cross-linking, cystic fibrosis transmembrane conductance regulator (CFTR), ion channel, phosphorylation, spectroscopy, synchrotron radiation circular dichroism
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Depositing User: Administrator
    Date Deposited: 10 Mar 2017 14:21
    Last Modified: 28 Sep 2018 12:23
    URI: http://eprints.bbk.ac.uk/id/eprint/18322

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