BIROn - Birkbeck Institutional Research Online

    Reconstitution of a minimal machinery capable of assembling periplasmic type IV pili

    Goosens, V.J. and Busch, Andreas and Georgiadou, M. and Castagnini, M. and Forest, K.T. and Waksman, Gabriel and Pelicic, V. (2017) Reconstitution of a minimal machinery capable of assembling periplasmic type IV pili. Proceedings of the National Academy of Sciences of the United States of America 114 (25), E4978-E4986. ISSN 0027-8424.

    [img]
    Preview
    Text
    19382.pdf - Published Version of Record
    Available under License Creative Commons Attribution Non-commercial.

    Download (1MB) | Preview

    Abstract

    Type IV pili (Tfp), which are key virulence factors in many bacterial pathogens, define a large group of multipurpose filamentous nanomachines widespread in Bacteria and Archaea. Tfp biogenesis is a complex multistep process, which relies on macromolecular assemblies composed of 15 conserved proteins in model gram- negative species. To improve our limited understanding of the molecular mechanisms of filament assembly, we have used a synthetic biology approach to reconstitute, in a nonnative heter- ologous host, a minimal machinery capable of building Tfp. Here we show that eight synthetic genes are sufficient to promote filament assembly and that the corresponding proteins form a macromolecular complex at the cytoplasmic membrane, which we have purified and characterized biochemically. Our results con- tribute to a better mechanistic understanding of the assembly of remarkable dynamic filaments nearly ubiquitous in prokaryotes.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): type IV pili, type IV filamentous nanomachines, filament assembly, synthetic biology
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Depositing User: Administrator
    Date Deposited: 14 Aug 2017 13:29
    Last Modified: 14 Aug 2017 13:45
    URI: http://eprints.bbk.ac.uk/id/eprint/19382

    Statistics

    Downloads
    Activity Overview
    56Downloads
    58Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item