Escherichia coli K1 RS218 Interacts with Human Brain Microvascular Endothelial Cells via Type 1 Fimbria Bacteria in the Fimbriated State
Teng, C.H. and Cai, M. and Shin, S. and Xie, Y. and Kim, K.J. and Khan, Naveed Ahmed and di Cello, F. and Kim, K.S. (2005) Escherichia coli K1 RS218 Interacts with Human Brain Microvascular Endothelial Cells via Type 1 Fimbria Bacteria in the Fimbriated State. Infection and Immunity 73 (5), pp. 2923-2931. ISSN 0019-9567.
Escherichia coli K1 is a major gram-negative organism causing neonatal meningitis. E. coli K1 binding to and invasion of human brain microvascular endothelial cells (HBMEC) are a prerequisite for E. coli penetration into the central nervous system in vivo. In the present study, we showed using DNA microarray analysis that E. coli K1 associated with HBMEC expressed significantly higher levels of the fim genes compared to nonassociated bacteria. We also showed that E. coli K1 binding to and invasion of HBMEC were significantly decreased with its fimH deletion mutant and type 1 fimbria locked-off mutant, while they were significantly increased with its type 1 fimbria locked-on mutant. E. coli K1 strains associated with HBMEC were predominantly type 1 fimbria phase-on (i.e., fimbriated) bacteria. Taken together, we showed for the first time that type 1 fimbriae play an important role in E. coli K1 binding to and invasion of HBMEC and that type 1 fimbria phase-on E. coli is the major population interacting with HBMEC.
|Additional Information:||Copyright © 2005, American Society for Microbiology. All Rights Reserved.|
|School:||Birkbeck Schools and Departments > School of Science > Biological Sciences|
|Date Deposited:||17 Aug 2005|
|Last Modified:||17 Apr 2013 12:32|
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