BIROn - Birkbeck Institutional Research Online

    Escherichia coli K1 RS218 Interacts with Human Brain Microvascular Endothelial Cells via Type 1 Fimbria Bacteria in the Fimbriated State

    Teng, C.H. and Cai, M. and Shin, S. and Xie, Y. and Kim, K.J. and Khan, Naveed Ahmed and di Cello, F. and Kim, K.S. (2005) Escherichia coli K1 RS218 Interacts with Human Brain Microvascular Endothelial Cells via Type 1 Fimbria Bacteria in the Fimbriated State. Infection and Immunity 73 (5), pp. 2923-2931. ISSN 0019-9567.

    [img]
    Preview
    Text
    khan7.pdf

    Download (226kB) | Preview

    Abstract

    Escherichia coli K1 is a major gram-negative organism causing neonatal meningitis. E. coli K1 binding to and invasion of human brain microvascular endothelial cells (HBMEC) are a prerequisite for E. coli penetration into the central nervous system in vivo. In the present study, we showed using DNA microarray analysis that E. coli K1 associated with HBMEC expressed significantly higher levels of the fim genes compared to nonassociated bacteria. We also showed that E. coli K1 binding to and invasion of HBMEC were significantly decreased with its fimH deletion mutant and type 1 fimbria locked-off mutant, while they were significantly increased with its type 1 fimbria locked-on mutant. E. coli K1 strains associated with HBMEC were predominantly type 1 fimbria phase-on (i.e., fimbriated) bacteria. Taken together, we showed for the first time that type 1 fimbriae play an important role in E. coli K1 binding to and invasion of HBMEC and that type 1 fimbria phase-on E. coli is the major population interacting with HBMEC.

    Metadata

    Item Type: Article
    Additional Information: Copyright © 2005, American Society for Microbiology. All Rights Reserved.
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Depositing User: Administrator
    Date Deposited: 17 Aug 2005
    Last Modified: 17 Apr 2013 12:32
    URI: http://eprints.bbk.ac.uk/id/eprint/204

    Statistics

    Downloads
    Activity Overview
    630Downloads
    468Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item