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    Structure of yeast cytochrome c oxidase in a supercomplex with cytochrome bc1

    Hartley, Andrew and Lukoyanova, Natalya and Zhang, Y. and Cabrera-Orefice, A. and Arnold, S. and Meunier, B. and Pinotsis, Nikos and Marechal, Amandine (2018) Structure of yeast cytochrome c oxidase in a supercomplex with cytochrome bc1. Nature Structural & Molecular Biology 26 , pp. 78-83. ISSN 1545-9993.

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    Abstract

    Cytochrome c oxidase (complex IV, CIV) is known in mammals to exist independently or in association with other respiratory proteins to form supercomplexes (SCs). In Saccharomyces cerevisiae, CIV is found solely in a SC with cytochrome bc1 (complex III, CIII). Here, we present the cryo-EM structure of S. cerevisiae CIV in a III2IV2 SC at 3.3 Å resolution. While overall similarity to mammalian homologues is high, we found notable differences in the supernumerary subunits Cox26 and Cox13; the latter exhibits a unique arrangement that precludes CIV dimerization as seen in bovine. A conformational shift in the matrix domain of Cox5A – involved in allosteric inhibition by ATP – may arise from its association with CIII. The CIII–CIV arrangement highlights a conserved interaction interface of CIII, albeit one occupied by complex I in mammalian respirasomes. We discuss our findings in the context of the potential impact of SC formation on CIV regulation.

    Metadata

    Item Type: Article
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Depositing User: Dr Amandine Marechal
    Date Deposited: 07 Jan 2019 08:39
    Last Modified: 08 Jul 2019 00:10
    URI: http://eprints.bbk.ac.uk/id/eprint/25364

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