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    Visualising membrane trafficking through the electron microscope: cryo-tomography of coat complexes

    Markova, Evgenia A. and Zanetti, Giulia (2019) Visualising membrane trafficking through the electron microscope: cryo-tomography of coat complexes. Acta Crystallographica Section D: Structural Biology , ISSN 2059-7983.

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    Abstract

    Coat proteins mediate vesicular transport between intracellular compartments, which is essential for the distribution of molecules within the eukaryotic cell. The global arrangement of coat proteins on the membrane is key to their function, and cryo-electron tomography and subtomogram averaging have been used to study membrane-bound coat proteins, providing crucial structural insight. This review outlines a workflow for the structural elucidation of coat proteins, incorporating recent developments in the collection and processing of cryo-electron tomography data. Recent work on coat protein I, coat protein II and retromer performed on in vitro reconstitutions or in situ is summarized. These studies have answered long-standing questions regarding the mechanisms of membrane binding, polymerization and assembly regulation of coat proteins.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): cryo-electron tomography, three-dimensional reconstruction, coat proteins, vesicular transport, COPII, subtomogram averaging
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Giulia Zanetti
    Date Deposited: 02 May 2019 09:21
    Last Modified: 29 Jul 2019 01:01
    URI: http://eprints.bbk.ac.uk/id/eprint/27394

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