Uracil-DNA glycosylase activities in hyperthermophilic micro-organisms

Koulis, A. and Cowan, D.A. and Pearl, L.H. and Savva, Renos (1996) Uracil-DNA glycosylase activities in hyperthermophilic micro-organisms. FEMS Microbiology Letters 143 (2-3), pp. 267-271. ISSN 0378-1097.

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Official URL: https://doi.org/10.1111/j.1574-6968.1996.tb08491.x

Abstract

Hyperthermophiles exist in conditions which present an increased threat to the informational integrity of their DNA, particularly by hydrolytic damage. As in mesophilic organisms, specific activities must exist to restore and protect this template function of DNA. In this study we have demonstrated the presence of thermally stable uracil-DNA glycosylase activities in seven hyperthermophiles; one bacterial: Thermotoga maritima, and six archaeal: Sulfolobus solfataricus, Sulfolobus shibatae, Sulfolobus acidocaldarius, Thermococcus litoralis, Pyrococcus furiosus and Pyrobaculum islandicum. Uracil-DNA glycosylase inhibitor protein of the Bacillus subtilis bacteriophage PBS1 shows activity against all of these, suggesting a highly conserved tertiary structure between hyperthermophilic and mesophilic uracil-DNA glycosylases.

Metadata

Item Type:	Article
School:	Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
Research Centres and Institutes:	Innovation Management Research, Birkbeck Centre for
Depositing User:	Sarah Hall
Date Deposited:	14 May 2019 14:48
Last Modified:	02 Aug 2023 17:51
URI:	https://eprints.bbk.ac.uk/id/eprint/27528

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