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    Gas phase stability of Protein Ions in a Cyclic Ion Mobility Spectrometry Traveling Wave Device

    Eldrid, C. and Ujma, J. and Kalfas, S. and Tomczyk, N. and Giles, K. and Morris, M. and Thalassinos, Kostas (2019) Gas phase stability of Protein Ions in a Cyclic Ion Mobility Spectrometry Traveling Wave Device. Analytical Chemistry 91 (12), pp. 7554-7561. ISSN 1520-6882.

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    Abstract

    Ion mobility mass spectrometry (IM-MS) allows separation of native protein ions into “conformational families”. Increasing the IM resolving power should allow finer structural information to be obtained and can be achieved by increasing the length of the IM separator. This, however, increases the time that protein ions spend in the gas phase and previous experiments have shown that the initial conformations of small proteins can be lost within tens of milliseconds. Here, we report on investigations of protein ion stability using a multipass traveling wave (TW) cyclic IM (cIM) device. Using this device, minimal structural changes were observed for Cytochrome C after hundreds of milliseconds, while no changes were observed for a larger multimeric complex (Concanavalin A). The geometry of the instrument (Q-cIM-ToF) also enables complex tandem IM experiments to be performed, which were used to obtain more detailed collision-induced unfolding pathways for Cytochrome C. The instrument geometry provides unique capabilities with the potential to expand the field of protein analysis via IM-MS.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): Analytical Chemistry
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    SWORD Depositor: Mr Joe Tenant
    Depositing User: Mr Joe Tenant
    Date Deposited: 20 Jun 2019 05:55
    Last Modified: 29 Jul 2019 01:30
    URI: http://eprints.bbk.ac.uk/id/eprint/27842

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