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Cavitation of liquid flow in electrospray mass spectrometry especially for addition of internal calibrant in ‘accurate mass’ measurements

Lord, Gwyn A. and Lim, Chang-Kee (2010) Cavitation of liquid flow in electrospray mass spectrometry especially for addition of internal calibrant in ‘accurate mass’ measurements. Rapid Communications in Mass Spectrometry 25 (1), pp. 50-54. ISSN 0951-4198.

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Official URL: http://dx.doi.org/10.1002/rcm.4828

Abstract

‘Accurate mass’ measurements in electrospray mass spectrometry are becoming more prevalent with the increasing availability of mass spectrometers with sufficient resolution. A reference compound is ideally admitted separately but almost simultaneously with the analyte and this is achieved by use of a ‘dual sprayer’ or voltage switching between reference and sample sprayers. This paper describes a novel third method, relying on cavitation (segmentation) of the liquid stream containing the reference compound, allowing the sample to ionise independently from the sample, thus preventing interference. The technique may also find application in kinetic experiments, such as protein folding studies. A high-performance liquid chromatography (HPLC) injector was also used to provide a reference compound, producing Gaussian-shaped profiles of varying ion intensity, thus allowing easier selection of a desirable measurement point where the intensities of the reference and analyte were similar.

Item Type: Article
School or Research Centre: Birkbeck Schools and Research Centres > School of Science > Biological Sciences
Depositing User: Administrator
Date Deposited: 27 Jan 2011 10:28
Last Modified: 17 Apr 2013 12:20
URI: http://eprints.bbk.ac.uk/id/eprint/2971

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