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4.6Å Cryo-EM reconstruction of tobacco mosaic virus from images recorded at 300keV on a 4k×4k CCD camera

Clare, Daniel K. and Orlova, Elena V. (2010) 4.6Å Cryo-EM reconstruction of tobacco mosaic virus from images recorded at 300keV on a 4k×4k CCD camera. Journal of Structural Biology 171 (3), pp. 303-308. ISSN 1047-8477.

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Official URL: http://dx.doi.org/10.1016/j.jsb.2010.06.011

Abstract

Tobacco mosaic virus (TMV) is a plant virus with a highly ordered organisation and has been described in three different structural states: As stacked disks without RNA (X-ray crystallography), as a helical form with RNA (X-ray fibre diffraction) and as a second distinct helical form with RNA (cryo-EM). Here we present a structural analysis of TMV as a test object to assess the quality of cryo-EM images recorded at 300 keV on a CCD camera. The 4.6 Å TMV structure obtained is consistent with the previous cryo-EM structure and confirms that there is a second helical form of TMV. The structure here also shows that with a similar number of TMV segments an equivalent resolution can be achieved with a 4k CCD camera at 300 keV.

Item Type: Article
Additional Information: Available from PubMed Central via Gold Open Access at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2939825/?tool=pmcentrez
Keyword(s) / Subject(s): TMV, Cryo-EM, helical processing, single particle processing and CCD camera
School or Research Centre: Birkbeck Schools and Research Centres > School of Science > Biological Sciences
Depositing User: Administrator
Date Deposited: 07 Feb 2011 11:07
Last Modified: 17 Apr 2013 12:20
URI: http://eprints.bbk.ac.uk/id/eprint/3044

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