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Crystallization and preliminary crystallographic analysis of theClostridium perfringensenterotoxin

Briggs, David C. and Smedley, J.G. and McClane, B.A. and Basak, Ajit K. (2010) Crystallization and preliminary crystallographic analysis of theClostridium perfringensenterotoxin. Acta Crystallographica Section F Structural Biology and Crystallization Communications 66 (7), pp. 794-797. ISSN 1744-3091.

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Official URL: http://dx.doi.org/10.1107/S1744309110016507

Abstract

Clostridium perfringens is a Gram-positive anaerobic species of bacterium that is notable for its ability to produce a plethora of toxins, including membrane-active toxins ([alpha]-toxins), pore-forming toxins ([epsilon]-toxins) and binary toxins ([iota]-toxins). Here, the crystallization of the full-length wild-type C. perfringens enterotoxin is reported, which is the causative agent of the second most prevalent food-borne illness in the United States and has been implicated in many other gastrointestinal pathologies. Several crystal forms were obtained. However, only two of these optimized crystal forms (I and II) were useable for X-ray diffraction data collection. The form I crystals diffracted to dmin = 2.7 Å and belonged to space group C2, while the form II crystals diffracted to dmin = 4 Å and belonged to space group P213.

Item Type: Article
Keyword(s) / Subject(s): Pore-forming toxins, enterotoxins, Clostridium perfringens
School or Research Centre: Birkbeck Schools and Research Centres > School of Science > Biological Sciences
Depositing User: Administrator
Date Deposited: 17 Feb 2011 09:37
Last Modified: 17 Apr 2013 12:20
URI: http://eprints.bbk.ac.uk/id/eprint/3070

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