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    Crystallization and preliminary crystallographic analysis of theClostridium perfringensenterotoxin

    Briggs, David C. and Smedley, J.G. and McClane, B.A. and Basak, Ajit K. (2010) Crystallization and preliminary crystallographic analysis of theClostridium perfringensenterotoxin. Acta Crystallographica Section F Structural Biology and Crystallization Communications 66 (7), pp. 794-797. ISSN 1744-3091.

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    Abstract

    Clostridium perfringens is a Gram-positive anaerobic species of bacterium that is notable for its ability to produce a plethora of toxins, including membrane-active toxins ([alpha]-toxins), pore-forming toxins ([epsilon]-toxins) and binary toxins ([iota]-toxins). Here, the crystallization of the full-length wild-type C. perfringens enterotoxin is reported, which is the causative agent of the second most prevalent food-borne illness in the United States and has been implicated in many other gastrointestinal pathologies. Several crystal forms were obtained. However, only two of these optimized crystal forms (I and II) were useable for X-ray diffraction data collection. The form I crystals diffracted to dmin = 2.7 Å and belonged to space group C2, while the form II crystals diffracted to dmin = 4 Å and belonged to space group P213.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): Pore-forming toxins, enterotoxins, Clostridium perfringens
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Depositing User: Administrator
    Date Deposited: 17 Feb 2011 09:37
    Last Modified: 17 Apr 2013 12:20
    URI: http://eprints.bbk.ac.uk/id/eprint/3070

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