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Mechanism of eIF6-mediated inhibition of ribosomal subunit joining

Gartmann, M. and Blau, M. and Armache, J. P. and Mielke, T. and Topf, Maya and Beckmann, R. (2010) Mechanism of eIF6-mediated inhibition of ribosomal subunit joining. Journal of Biological Chemistry 285 (20), pp. 14848-14851. ISSN 0021-9258.

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Official URL: http://dx.doi.org/10.1074/jbc.C109.096057

Abstract

During the process of ribosomal assembly, the essential eukaryotic translation initiation factor 6 (eIF6) is known to act as a ribosomal anti-association factor. However, a molecular understanding of the anti-association activity of eIF6 is still missing. Here we present the cryo-electron microscopy reconstruction of a complex of the large ribosomal subunit with eukaryotic eIF6 from Saccharomyces cerevisiae. The structure reveals that the eIF6 binding site involves mainly rpL23 (L14p in Escherichia coli). Based on our structural data, we propose that the mechanism of the anti-association activity of eIF6 is based on steric hindrance of intersubunit bridge formation around the dynamic bridge B6.

Item Type: Article
Keyword(s) / Subject(s): Electron Microscopy (EM), Ribosome Assembly, Ribosome Function, Ribosome Structure, Ribosomes
School or Research Centre: Birkbeck Schools and Research Centres > School of Science > Biological Sciences
Depositing User: Administrator
Date Deposited: 23 Feb 2011 14:53
Last Modified: 17 Apr 2013 12:20
URI: http://eprints.bbk.ac.uk/id/eprint/3134

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