Bereswill, S. and Mullen, Lisa M. and Bossé, J.T. and Nair, S.P. and Ward, J.M. and Rycroft, A.N. and Robertson, Giles and Langford, P.R. and Henderson, B. (2008) Pasteurellaceae ComE1 proteins combine the properties of Fibronectin Adhesins and DNA binding competence proteins. PLoS One 3 (12), ISSN 1932-6203.
Text (Published draft (Refereed))
Available under License Creative Commons Attribution.
Download (709kB) | Preview
A novel fibronectin-binding protein from Pasteurella multocida (PM1665) that binds to the fibronectin type III9-10 modules via two helix-hairpin-helix motifs has recently been described . This protein shares homology with competence-related DNA-binding and uptake proteins (ComEA and ComE) from Gram-positive and Gram-negative bacteria. Here, we show that recombinant PM1665 (now designated ComE1) also binds to DNA through the same helix-hairpin-helix motifs required for fibronectin-binding. This binding to DNA is non sequence-specific and is confined to double-stranded DNA. We have cloned and expressed ComE1 proteins from five members of the Pasteurellaceae in order to further investigate the function(s) of these proteins. When expressed as recombinant GST-fusion proteins, all of the homologues bound both to fibronectin and to double-stranded DNA. Inactivation of the gene encoding the ComE1 homologue in Actinobacillus pleuropneumoniae indicates major roles for these proteins in at least two processes: natural transformation, and binding of bacteria to fibronectin.
|School or Research Centre:||Birkbeck Schools and Research Centres > School of Science > Biological Sciences|
|Date Deposited:||24 Feb 2011 10:25|
|Last Modified:||17 Apr 2013 12:33|
Archive Staff Only (login required)