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Mammal-restricted elements predispose human RET to folding impairment by HSCR mutations

Kjaer, S. and Hanrahan, S. and Totty, N. and McDonald, Neil Q. (2010) Mammal-restricted elements predispose human RET to folding impairment by HSCR mutations. Nature Structural & Molecular Biology 17 (6), pp. 726-731. ISSN 1545-9993.

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Official URL: http://dx.doi.org/10.1038/nsmb.1808

Abstract

The maturation of human RET is adversely affected by a range of missense mutations found in patients with Hirschsprung's disease (HSCR), a complex multigenic disease. Here we show that two N-terminal cadherin-like domains, CLD1 and CLD2 (CLD(1-2)), from human RET adopt a clam-shell arrangement distinct from that of classical cadherins. CLD1 structural elements and disulfide composition are unique to mammals, indicating an unexpected structural diversity within higher and lower vertebrate RET CLD regions. We identify two unpaired cysteines that predispose human RET to maturation impediments in the endoplasmic reticulum and establish a quantitative cell-based RET maturation assay that offers a biochemical correlate of HSCR disease severity. Our findings provide a key conceptual framework and means of testing and predicting genotype-phenotype correlations in HSCR.

Item Type: Article
School or Research Centre: Birkbeck Schools and Research Centres > School of Science > Biological Sciences
Depositing User: Administrator
Date Deposited: 30 Mar 2011 14:57
Last Modified: 17 Apr 2013 12:20
URI: http://eprints.bbk.ac.uk/id/eprint/3221

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