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RNA channelling by the eukaryotic exosome

Malet, Hélène and Topf, Maya and Clare, Daniel K. and Ebert, J. and Bonneau, F. and Basquin, J. and Drazkowska, K. and Tomecki, R. and Dziembowski, A. and Conti, E. and Saibil, Helen R. and Lorentzen, Esben (2010) RNA channelling by the eukaryotic exosome. EMBO Reports 11 (12), pp. 936-942. ISSN 1469-221X.

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The eukaryotic exosome is a key nuclease for the degradation, processing and quality control of a wide variety of RNAs. Here, we report electron microscopic reconstructions and pseudo-atomic models of the ten-subunit Saccharomyces cerevisiae exosome in the unbound and RNA-bound states. In the RNA-bound structures, extra density that is visible at the entry and exit sites of the exosome channel indicates that a substrate-threading mechanism is used by the eukaryotic exosome. This channelling mechanism seems to be conserved in exosome-like complexes from all domains of life, and might have been present in the most recent common ancestor.

Item Type: Article
Keyword(s) / Subject(s): exosome, RNA degradation, cryo-electron microscope, macromolecular complex
School or Research Centre: Birkbeck Schools and Research Centres > School of Science > Biological Sciences
Depositing User: Administrator
Date Deposited: 01 Apr 2011 10:35
Last Modified: 11 Oct 2016 12:01

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