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The use of high-resolution (1)H nuclear magnetic resonance (NMR) spectroscopy in the clinical diagnosis of Acanthamoeba

Hauber, Sandra and Parkes, H. and Siddiqui, R. and Khan, Naveed Ahmed (2011) The use of high-resolution (1)H nuclear magnetic resonance (NMR) spectroscopy in the clinical diagnosis of Acanthamoeba. Parasitology Research 109 (6), pp. 1661-1669. ISSN 0932-0113.

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Official URL: http://dx.doi.org/10.1007/s00436-011-2439-8

Abstract

Acanthamoeba are opportunistic protozoan pathogens that can produce sight-threatening keratitis and fatal granulomatous encephalitis. The successful prognosis requires early diagnosis and differentiation of pathogenic Acanthamoeba spp. followed by aggressive treatment regimen. In this study, we tested the use of high-resolution (1)H NMR spectroscopy in the clinical diagnosis of Acanthamoeba. Using NMR spectroscopy combined with Pattern Recognition Analysis (PRA), we analysed variations in the biochemical 'fingerprint' of invasive and non-invasive Acanthamoeba, its closely related genus, Balamuthia mandrillaris, neuropathogenic Escherichia coli K1 strain E44, a laboratory strain of E. coli K-12, HB101, mammalian cells including human brain microvascular endothelial cells and monkey kidney cells. The findings revealed significant variations in the metabolites of amoebae, mammalian cells and bacteria. Interestingly, (1)H NMR spectra provided distinct biochemical profiles of clinical and non-clinical Acanthamoeba isolates highlighting the potential of (1)H NMR spectroscopy combined with PRA for the development of a novel diagnostic test that could rapidly identify pathogenic Acanthamoeba isolates with high sensitivity and specificity.

Item Type: Article
School or Research Centre: Birkbeck Schools and Research Centres > School of Science > Biological Sciences
Depositing User: Administrator
Date Deposited: 23 May 2011 09:45
Last Modified: 17 Apr 2013 12:20
URI: http://eprints.bbk.ac.uk/id/eprint/3336

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