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    The structure of a resuscitation-promoting factor domain from Mycobacterium tuberculosis shows homology to lysozymes

    Cohen Gonsaud, M. and Barthe, P. and Bagneris, Claire and Henderson, B. and Ward, J.M. and Roumestand, C. and Keep, Nicholas H. (2005) The structure of a resuscitation-promoting factor domain from Mycobacterium tuberculosis shows homology to lysozymes. Nature Structural and Molecular Biology 12 (3), pp. 270-273. ISSN 1545-9993.

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    Abstract

    Resuscitation-promoting factor (RPF) proteins reactivate stationary-phase cultures of (G+C)-rich Gram-positive bacteria including the causative agent of tuberculosis, Mycobacterium tuberculosis. We report the solution structure of the RPF domain from M. tuberculosis Rv1009 (RpfB) solved by heteronuclear multidimensional NMR. Structural homology with various glycoside hydrolases suggested that RpfB cleaved oligosaccharides. Biochemical studies indicate that a conserved active site glutamate is important for resuscitation activity. These data, as well as the presence of a clear binding pocket for a large molecule, indicate that oligosaccharide cleavage is probably the signal for revival from dormancy.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): RPF, NMR, Resuscitation promoting factors, Mycobacterium tuberculosis, dormant culture, bacterial cytokine
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 22 Mar 2006
    Last Modified: 06 Dec 2016 10:42
    URI: http://eprints.bbk.ac.uk/id/eprint/343

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