BIROn - Birkbeck Institutional Research Online

The GDP-GTP exchange factor collybistin: an essential determinant of neuronal gephyrin clustering

Harvey, K. and Duguid, I.C. and Alldred, M.J. and Beatty, S.E. and Ward, H. and Keep, Nicholas H. and Lingenfelter, S.E. and Pearce, B.R. and Lundgren, J.D. and Owens, Max J. and Smart, T.G. and Luscher, B. and Rees, M.I. and Harvey, R.J. (2004) The GDP-GTP exchange factor collybistin: an essential determinant of neuronal gephyrin clustering. The Journal of Neuroscience 24 (25), pp. 5816-5826. ISSN 0270-6474.

[img]
Preview
Text
Binder1.pdf

Download (594Kb) | Preview
Official URL: http://dx.doi.org/10.1523/JNEUROSCI.1184-04.2004

Abstract

Glycine receptors (GlyRs) and specific subtypes of GABA(A) receptors are clustered at synapses by the multidomain protein gephyrin, which in turn is translocated to the cell membrane by the GDP-GTP exchange factor collybistin. We report the characterization of several new variants of collybistin, which are created by alternative splicing of exons encoding an N-terminal src homology 3 (SH3) domain and three alternate C termini (CB1, CB2, and CB3). The presence of the SH3 domain negatively regulates the ability of collybistin to translocate gephyrin to submembrane microaggregates in transfected mammalian cells. Because the majority of native collybistin isoforms appear to harbor the SH3 domain, this suggests that collybistin activity may be regulated by protein-protein interactions at the SH3 domain. We localized the binding sites for collybistin and the GlyR beta subunit to the C-terminal MoeA homology domain of gephyrin and show that multimerization of this domain is required for collybistin-gephyrin and GlyR-gephyrin interactions. We also demonstrate that gephyrin clustering in recombinant systems and cultured neurons requires both collybistin-gephyrin interactions and an intact collybistin pleckstrin homology domain. The vital importance of collybistin for inhibitory synaptogenesis is underlined by the discovery of a mutation (G55A) in exon 2 of the human collybistin gene (ARHGEF9) in a patient with clinical symptoms of both hyperekplexia and epilepsy. The clinical manifestation of this collybistin missense mutation may result, at least in part, from mislocalization of gephyrin and a major GABA(A) receptor subtype.

Item Type: Article
Additional Information: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in The Journal of Neuroscience following peer review. The definitive publisher-authenticated version as cited above is available online at http://dx.doi.org/10.1523/JNEUROSCI.1184-04.2004
Keyword(s) / Subject(s): dendritic transport, epilepsy, GABAA receptor, glycine receptor, hyperekplexia, trafficking
School or Research Centre: Birkbeck Schools and Research Centres > School of Science > Biological Sciences
Depositing User: Sandra Plummer
Date Deposited: 17 May 2006
Last Modified: 17 Apr 2013 12:32
URI: http://eprints.bbk.ac.uk/id/eprint/372

Archive Staff Only (login required)

Edit/View Item Edit/View Item