Investigation of the complexation of the anti-cancer drug novantrone with the hairpin structure of the deoxyheptanucleotide 5'-d(GpCpGpApApGpC)
Kostjukov, V.V. and Pahomov, V.I. and Andrejuk, D.D. and Davies, David B. and Evstigneev, M.P. (2007) Investigation of the complexation of the anti-cancer drug novantrone with the hairpin structure of the deoxyheptanucleotide 5'-d(GpCpGpApApGpC). Journal of Molecular Structure 843 (1-3), 78 - 86. ISSN 0022-2860.
In aqueous solution the deoxyheptanucleotide, 5′-d(GpCpGpApApGpC), exists as a very stable hairpin structure in equilibrium with small proportions of the single-stranded and duplex forms. Complexation of the anti-cancer drug novantrone (mitoxantrone) with the DNA heptamer was investigated by one- and two-dimensional 500 MHz 1H NMR spectroscopy (2M-TOCSY, 2M-NOESY) and molecular dynamics simulations. The proton chemical shifts of NOV in mixed solutions with the heptamer were measured as a function of concentration and temperature and the equilibrium association parameters were determined for complexation of NOV with the three forms of the heptamer. The spatial structure of the complex of the antibiotic with the hairpin form of the heptamer was built on the basis of 2D-NOE data. The conformational dynamics of the complex and its interaction with the water environment were investigated by molecular dynamics methods. The results suggest that NOV complexes with the hairpin form of the heptamer in solution by intercalation. Complexation of NOV with the hairpin stem results in a disruption of about one half of the intramolecular water bridges of the hairpin, which is considered to be the main reason for the observed decrease in the thermodynamical stability of the hairpin on binding with the ligand.
|Keyword(s) / Subject(s):||DNA hairpin, Novantrone, intercalation, NMR spectroscopy, molecular dynamics|
|School:||Birkbeck Schools and Departments > School of Science > Biological Sciences|
|Date Deposited:||08 Aug 2011 12:51|
|Last Modified:||17 Apr 2013 12:21|
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