BIROn - Birkbeck Institutional Research Online

    Topologies of a Substrate Protein Bound to the Chaperonin GroEL

    Elad, Nadav and Farr, G.W. and Clare, Daniel K. and Orlova, Elena and Horwich, A.L. and Saibil, Helen (2007) Topologies of a Substrate Protein Bound to the Chaperonin GroEL. Molecular Cell 26 (3), 415 - 426. ISSN 1097-2765.

    Full text not available from this repository.

    Abstract

    The chaperonin GroEL assists polypeptide folding through sequential steps of binding nonnative protein in the central cavity of an open ring, via hydrophobic surfaces of its apical domains, followed by encapsulation in a hydrophilic cavity. To examine the binding state, we have classified a large data set of GroEL binary complexes with nonnative malate dehydrogenase (MDH), imaged by cryo-electron microscopy, to sort them into homogeneous subsets. The resulting electron density maps show MDH associated in several characteristic binding topologies either deep inside the cavity or at its inlet, contacting three to four consecutive GroEL apical domains. Consistent with visualization of bound polypeptide distributed over many parts of the central cavity, disulfide crosslinking could be carried out between a cysteine in a bound substrate protein and cysteines substituted anywhere inside GroEL. Finally, substrate binding induced adjustments in GroEL itself, observed mainly as clustering together of apical domains around sites of substrate binding.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): Proteins
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 08 Aug 2011 10:26
    Last Modified: 06 Dec 2016 11:14
    URI: http://eprints.bbk.ac.uk/id/eprint/3977

    Statistics

    Downloads
    Activity Overview
    0Downloads
    903Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item