BIROn - Birkbeck Institutional Research Online

    Atypical AAA+ subunit packing creates an expanded cavity for disaggregation by the protein-remodeling factor Hsp104

    Wendler, Petra and Shorter, J. and Plisson, Celia and Cashikar, A.G. and Lindquist, S. and Saibil, Helen R. (2007) Atypical AAA+ subunit packing creates an expanded cavity for disaggregation by the protein-remodeling factor Hsp104. Cell 131 (7), 1366 - 1377. ISSN 0092-8674.

    Full text not available from this repository.

    Abstract

    Hsp104, a yeast protein-remodeling factor of the AAA+ (ATPases associated with various cellular activities) superfamily, and its homologs in bacteria and plants mediate cell recovery after severe stress by disaggregating denatured proteins through a poorly understood mechanism. Here, we present cryo-electron microscopy maps and domain fitting of Hsp104 hexamers, revealing an unusual arrangement of AAA+ modules with the prominent coiled-coil domain intercalated between the AAA+ domains. This packing results in a greatly expanded cavity, which is capped at either end by N- and C-terminal domains. The fitted structures as well as mutation of conserved coiled-coil arginines suggest that the coiled-coil domain plays a major role in the extraction of proteins from aggregates, providing conserved residues for key functions in ATP hydrolysis and potentially for substrate interaction. The large cavity could enable the uptake of polypeptide loops without a requirement for exposed N or C termini.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): Cellbio, proteins
    School: Birkbeck Faculties and Schools > Faculty of Science > School of Natural Sciences
    Research Centres and Institutes: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 08 Aug 2011 09:23
    Last Modified: 02 Aug 2023 16:55
    URI: https://eprints.bbk.ac.uk/id/eprint/3985

    Statistics

    Activity Overview
    6 month trend
    0Downloads
    6 month trend
    230Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item