Identification of PVK/CAP2b neuropeptides from single neurohemal organs of the stable fly and horn fly via MALDI-TOF/TOF tandem mass spectrometry
Nachman, R.J. and Russell, W. and Coast, Geoffrey M. and Russell, D. and Miller, J. and Predel, R. (2006) Identification of PVK/CAP2b neuropeptides from single neurohemal organs of the stable fly and horn fly via MALDI-TOF/TOF tandem mass spectrometry. Peptides 27 (3), pp. 521-526. ISSN 0196-9781.
MALDI-TOF/TOF tandem mass spectrometry has been applied to determine the complete sequences of the PVK/CAP2b neuropeptides in the stable fly Stomoxys calcitrans and horn fly Haematobia irritans, insect pests of livestock. This peptidomic analysis of single neurohemal organ preparations allows the unambiguous assignment of internal Leu/Ile positions not distinguishable by previous mass spectrometric techniques. The sequences are as follows: Stoca-PVK/CAP2b-1, AGGASGLYAFPRVa; Stoca-PVK/CAP2b-2, NAKLYPVPRVa; and Haeir-PVK/CAP2b-1, AGGASGLYAFPRVa; Haeir-PVK/CAP2b-1, NAKLYPMPRVa. Both Stoca-PVK/CAP2b-1 and -2 stimulate Malpighian tubule fluid secretion in the stable fly, with EC50 values between 3 and 11 nM. The identification of these novel neuropeptides adds to our knowledge of the peptidomes of flies, and can aid in the development of neuropeptide-based control strategies of these insect pests.
|Keyword(s) / Subject(s):||MALDI-TOF/TOF mass spectrometry, insect, neuropeptide, Periviscerokinin, CAP2b, stomoxys calcitrans, Haematobia irritans, peptidomics|
|School:||Birkbeck Schools and Departments > School of Science > Biological Sciences|
|Date Deposited:||18 Aug 2011 09:21|
|Last Modified:||17 Apr 2013 12:21|
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