BIROn - Birkbeck Institutional Research Online

    The influence of different lipid environments on the structure and function of the hepatitis C virus p7 ion channel protein

    Whitfield, T. and Miles, Andrew J. and Scheinost, J.C. and Offer, J. and Wentworth, P. and Dwek, R.A. and Wallace, Bonnie A. and Biggin, P.C. and Zitzmann, N. (2011) The influence of different lipid environments on the structure and function of the hepatitis C virus p7 ion channel protein. Molecular Membrane Biology 28 (5), pp. 254-264. ISSN 0968-7688.

    Full text not available from this repository.

    Abstract

    The hepatitis C virus (HCV) encodes the p7 protein that oligomerizes to form an ion channel. The 63 amino acid long p7 monomer is an integral membrane protein predominantly found in the endoplasmic reticulum (ER). Although it is currently unknown whether p7 is incorporated into secreted virions, its presence is crucial for the release of infectious virus. The molecular and biophysical mechanism employed by the p7 ion channel is largely unknown, but in vivo it is likely to be embedded in membranes undergoing changes in lipid composition. In this study we analyze the influence of the lipid environment on p7 ion channel structure and function using electrophysiology and synchrotron radiation circular dichroism (SRCD) spectroscopy. We incorporated chemically synthesized p7 polypeptides into artificial planar membranes of various lipid compositions. A lipid bilayer composition comprising phosphatidylcholine (PC) and phosphatidylethanolamine (PE) (4:1 PC:PE) led to burst-like patterns in the channel recordings with channel openings lasting up to 0.5 s. The reverse ratio of PC:PE (1:4) gave rise to individual channels continuously opening for up to 8 s. SRCD spectroscopy of p7 embedded into liposomes of corresponding lipid compositions suggests there is a structural effect of the lipid composition on the p7 protein.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): Planar lipid bilayers, ion channel, membrane proteins
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Bioinformatics, Bloomsbury Centre for, Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 15 Aug 2011 13:32
    Last Modified: 07 Dec 2016 15:05
    URI: http://eprints.bbk.ac.uk/id/eprint/4096

    Statistics

    Downloads
    Activity Overview
    0Downloads
    0Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item