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Isolation and characterization of free haem from the shell gland of quail and hen

Gorchein, Abel and Lord, Gwyn A. and Lim, Chang-Kee (2012) Isolation and characterization of free haem from the shell gland of quail and hen. Biomedical Chromatography 26 (3), pp. 355-357. ISSN 0269-3879.

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Official URL: http://dx.doi.org/10.1002/bmc.1665

Abstract

Free haem was isolated from the shell gland of the quail, Coturnix coturnix japonica, and of the fowl, Galinus domesticus, and characterized by HPLC-ESI-MS/MS. Quantification by HPLC gave values of 1.17-1.40 nmol/mg quail shell gland protein for haem, 1.66-2.17 nmol/mg protein for protoporphyrin and 0.25-0.40 nmol/mg protein for biliverdin. Possible implications of this previously unreported finding are discussed but they are not considered incompatible with the conclusion that all eggshell pigments are endogenously synthesized in the oviduct system. Copyright © 2011 John Wiley & Sons, Ltd.

Item Type: Article
Keyword(s) / Subject(s): haem, protoporphyrin, biliverdin, HPLC/ESI-MS/MS, shell gland, quail
School or Research Centre: Birkbeck Schools and Research Centres > School of Science > Biological Sciences
Depositing User: Administrator
Date Deposited: 15 Nov 2011 12:15
Last Modified: 17 Apr 2013 12:21
URI: http://eprints.bbk.ac.uk/id/eprint/4308

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