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Analysis of antigenically important residues in human influenza A virus in terms of B-cell epitopes

Lees, William D. and Moss, David S. and Shepherd, Adrian J. (2011) Analysis of antigenically important residues in human influenza A virus in terms of B-cell epitopes. Journal of Virology 85 (17), pp. 8548-8555. ISSN 0022-538X.

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In this paper we undertake an analysis of the antigenicity of influenza A virus hemagglutinin. We developed a novel computational approach to the identification of antigenically active regions and showed that the amino acid substitutions between successive predominant seasonal strains form clusters that are consistent, in terms of both their location and their size, with the properties of B-cell epitopes in general and with those epitopes that have been identified experimentally in influenza A virus hemagglutinin to date. Such an interpretation provides a biologically plausible framework for an understanding of the location of antigenically important substitutions that is more specific than the canonical "antigenic site" model and provides an effective basis for deriving models that predict antigenic escape in the H3N2 subtype. Our results support recent indications that antibodies binding to the "stalk" region of hemagglutinin are found in the human population and exert evolutionary pressure on the virus. Our computational approach provides a possible method for identifying antigenic escape through evolution in this region, which in some cases will not be identified by the hemagglutinin inhibition assay.

Item Type: Article
Keyword(s) / Subject(s): Amino Acid SubstitutionAntigens, Viral, Computational Biology, Epitopes, B-Lymphocyte, Hemagglutinin Glycoproteins, Influenza Virus, Humans, Immune Evasion Influenza A Virus, H1N1 Subtype, H3N2 Subtype, Models, Molecular, Mutation, Missense
School or Research Centre: Birkbeck Schools and Research Centres > School of Science > Biological Sciences
Depositing User: Administrator
Date Deposited: 15 Nov 2011 09:48
Last Modified: 10 Jun 2013 07:07

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