An analysis of NMR sensitivity enhancements obtained using non-uniform weighted sampling, and the application to protein NMR
Waudby, Christopher A. and Christodoulou, John (2012) An analysis of NMR sensitivity enhancements obtained using non-uniform weighted sampling, and the application to protein NMR. Journal of Magnetic Resonance 219 , pp. 46-52. ISSN 1090-7807.
Non-uniform weighted sampling (NUWS) is a sampling strategy, related to non-uniform sampling (NUS) in the limit of long acquisition times, in which each indirect increment of a multidimensional spectrum is sampled multiple times according to some weighting function. As the spectrum is fully sampled it can be processed in a conventional manner by the discrete Fourier transform, making the analysis of sensitivity much more straightforward than for NUS data. Previously, 2–3 fold increases in signal-to-noise ratio (SNR) have been reported using NUWS. However, as the sampling schedule acts as a window function, the observed SNR must be compared with uniformly sampled data apodized using the same weighting function. On doing this, we calculate more modest improvements of 10–20% in SNR, and these are verified experimentally for spectra of α-synuclein and YFP. Nevertheless, we prove that NUWS always improves the sensitivity compared with identically processed uniformly sampled data, and when combined with rapid recycling experiments such as the SOFAST-HMQC, NUWS methods have the potential to make a useful and practical contribution to sensitivity-limited measurements.
|Keyword(s) / Subject(s):||Nonuniform sampling, Sensitivity, Apodization, Window functions|
|School:||Birkbeck Schools and Departments > School of Science > Biological Sciences|
|Research Centre:||Structural Molecular Biology, Institute of (ISMB)|
|Date Deposited:||04 May 2012 13:23|
|Last Modified:||06 Dec 2016 10:48|
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