BIROn - Birkbeck Institutional Research Online

    An analysis of NMR sensitivity enhancements obtained using non-uniform weighted sampling, and the application to protein NMR

    Waudby, Christopher A. and Christodoulou, John (2012) An analysis of NMR sensitivity enhancements obtained using non-uniform weighted sampling, and the application to protein NMR. Journal of Magnetic Resonance 219 , pp. 46-52. ISSN 1090-7807.

    Full text not available from this repository.

    Abstract

    Non-uniform weighted sampling (NUWS) is a sampling strategy, related to non-uniform sampling (NUS) in the limit of long acquisition times, in which each indirect increment of a multidimensional spectrum is sampled multiple times according to some weighting function. As the spectrum is fully sampled it can be processed in a conventional manner by the discrete Fourier transform, making the analysis of sensitivity much more straightforward than for NUS data. Previously, 2–3 fold increases in signal-to-noise ratio (SNR) have been reported using NUWS. However, as the sampling schedule acts as a window function, the observed SNR must be compared with uniformly sampled data apodized using the same weighting function. On doing this, we calculate more modest improvements of 10–20% in SNR, and these are verified experimentally for spectra of α-synuclein and YFP. Nevertheless, we prove that NUWS always improves the sensitivity compared with identically processed uniformly sampled data, and when combined with rapid recycling experiments such as the SOFAST-HMQC, NUWS methods have the potential to make a useful and practical contribution to sensitivity-limited measurements.

    Metadata

    Item Type: Article
    Keyword(s) / Subject(s): Nonuniform sampling, Sensitivity, Apodization, Window functions
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 04 May 2012 13:23
    Last Modified: 06 Dec 2016 10:48
    URI: http://eprints.bbk.ac.uk/id/eprint/4748

    Statistics

    Downloads
    Activity Overview
    0Downloads
    117Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item