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Association of Neonicotinoid insensitivity with a conserved residue in the Loop D binding region of the Tick Nicotinic Acetylcholine Receptor

Erdmanis, L. and O’Reilly, Andrias O. and Williamson, M.S. and Field, L.M. and Turberg, A. and Wallace, Bonnie A. (2012) Association of Neonicotinoid insensitivity with a conserved residue in the Loop D binding region of the Tick Nicotinic Acetylcholine Receptor. Biochemistry 51 (23), pp. 4627-4629. ISSN 0006-2960.

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Official URL: http://dx.doi.org/10.1021/bi300522s

Abstract

Neonicotinoid insecticides target nicotinic acetylcholine receptors (nAChR) in the nervous system of insects but are largely ineffective against ticks. This study aimed to identify the molecular basis for this insensitivity. A homology model of the nAChR binding domain was generated on the basis of the crystal structure of an acetylcholine-binding protein with the insecticide imidacloprid bound. We hypothesized that tick β-subunits would differ at a critical residue (Arg81) in their D loops. To test this, we sequenced nAChR genes from five tick species and found that instead of the conserved arginine found in insects, a glutamine was present in all the tick sequences.

Item Type: Article
School or Research Centre: Birkbeck Schools and Research Centres > School of Science > Biological Sciences
Depositing User: Administrator
Date Deposited: 11 Jun 2012 09:39
Last Modified: 17 Apr 2013 12:23
URI: http://eprints.bbk.ac.uk/id/eprint/4787

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