BIROn - Birkbeck Institutional Research Online

    Chlamydiae assemble a pathogen synapse to hijack the host endoplasmic reticulum

    Dumoux, Maud and Clare, Daniel K. and Saibil, Helen R. and Hayward, Richard (2012) Chlamydiae assemble a pathogen synapse to hijack the host endoplasmic reticulum. Traffic 13 (12), pp. 1612-1617. ISSN 1398-9219.

    [img]
    Preview
    Text
    5038.pdf - Published Version of Record
    Available under License Creative Commons Attribution.

    Download (188MB) | Preview

    Abstract

    Chlamydiae are obligate intracellular bacterial pathogens that replicate within a specialised membrane-bound compartment, termed an ‘inclusion’. The inclusion membrane is a critical host-pathogen interface, yet the extent of its interaction with cellular organelles and the origin of this membrane remain poorly defined. Here we show that the host endoplasmic reticulum (ER) is specifically recruited to the inclusion, and that key rough ER (rER) proteins are enriched on and translocated into the inclusion. rER recruitment is a Chlamydia-orchestrated process that occurs independently of host trafficking. Generation of infectious progeny requires an intact ER, since ER vacuolation early during infection stalls inclusion development, whereas disruption post ER recruitment bursts the inclusion. Electron tomography and immunolabelling of Chlamydia-infected cells reveal ‘pathogen synapses’ at which ordered arrays of chlamydial type III secretion complexes connect to the inclusion membrane only at rER contact sites. Our data demonstrate a supramolecular assembly involved in pathogen hijack of a key host organelle.

    Metadata

    Item Type: Article
    School: Birkbeck Schools and Departments > School of Science > Biological Sciences
    Research Centre: Structural Molecular Biology, Institute of (ISMB)
    Depositing User: Administrator
    Date Deposited: 21 Aug 2012 09:09
    Last Modified: 06 Dec 2016 11:14
    URI: http://eprints.bbk.ac.uk/id/eprint/5038

    Statistics

    Downloads
    Activity Overview
    34Downloads
    138Hits

    Additional statistics are available via IRStats2.

    Archive Staff Only (login required)

    Edit/View Item Edit/View Item