Isolation and characterization of carbendazim degrading Trichoderma harzianum rifai mutants
Soares de Melo, I. and Maganhotto de Silva, C.M. and Nicklin, Jane (2010) Isolation and characterization of carbendazim degrading Trichoderma harzianum rifai mutants. Pesticidas: Revista de Ecotoxicologia e Meio Ambiente 20 (1), ISSN 1983-9847.
Mutants of Trichoderma harzianum Rifai, obtained after ultraviolet (UV) light exposure, showed high resistant to the fungicide benomyl. A mutant (2B6) was capable of degrading carbendazim, other fungicide of the benzimidazole fungicide. This mutant degraded 41.5% of the molecule within five days. This and others mutants (2B1 and 2B2) presented variation in size and frequency of uni-nucleated and/or bi-nucleated spores compared to the wild type. Four primers generated RAPDs patterns that allowed the mutant to be differentiated from the wild-type. It is concluded that using UV mutagenization, it is feasible to obtain strains of T. harzianum with improved pesticide degradation ability.
|School:||Birkbeck Schools and Departments > School of Science > Biological Sciences|
|Research Centre:||Structural Molecular Biology, Institute of (ISMB)|
|Date Deposited:||21 Nov 2012 16:31|
|Last Modified:||06 Dec 2016 11:25|
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